Catalogue
Sheep anti Mouse Bax-beta
Catalog number: X1290P$330.00
Add To CartIsotype | IgG |
Product Type |
Polyclonal Antibody |
Units | 250 µg |
Host | Sheep |
Species Reactivity |
Mouse |
Application |
Immunoprecipitation Western Blotting |
Background
Bax is encoded by a 1.5 kb RNA species created by the retention of intron 5 in the bax mRNA transcript. The exon4/intron5 splice junction is evolutionarily conserved and is found in both the human and mouse bax coding regions. The 630 bp intron 5 contributes 60 amino acids to the Bax protein before a stop codon is encountered during translation. The Bax protein is 24 kDa in size and although it contains the BH1, BH2, and BH3 domains identical to Bax, it has a unique carboxyl terminus and does not have a hydrophobic transmembrane domain (Oltvai et al., 1993). Bax mRNA has been found in many mouse tissues using RT-PCR. It is found co-expressed with Bax in lung, stomach, kidney, thymus, lymph node, bone marrow, pancreas, and spleen. Bax mRNA was very abundant in brain and duodenum with almost no Bax mRNA detected in these tissues (Oltvai et al., 1993).
Synonyms: Apoptosis regulator Bax cytoplasmic isoform beta , BaxB, BAX, BCL2 associated X protein, Apoptosis regulator BAX, BCL2 associated X protein isoform beta, Bax beta, Bax B
Source
Immunogen: Synthetic peptide corresponding to amino acids 172 to 199 of theBax protein
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Antibody detects Bax? protein by immunoprecipitation only. Will not detect denatured protein by Western blot. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Oltvai, Z.N., et al. Bcl-2 Heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 1993, 74, 609-619.
Protein Reference(s)
Database Name: UniProt
Accession Number: Q07812
Safety Datasheet(s) for this product:
EA_Sodium Azide |
" Immunoprecipitation of in vitro translated Baxα (1) and Baxβ (2) using Bax antibody, cln 6A7 (Cat. No. A105M), Baxα native (3) and denatured protein (4) using Baxβ antibody, and Baxβ native (5) and denatured (6) protein using Baxβ antibody."