Catalogue
Sheep anti Human TAK1
Catalog number: X1088P$319.00
Add To Cart| Isotype | IgG |
| Product Type |
Polyclonal Antibody |
| Units | 250 µg |
| Host | Sheep |
| Species Reactivity |
Human |
| Application |
Immunoprecipitation Western Blotting |
Background
C-TAK1 (Cdc25C associated protein kinase) is a ubiquitously expressed protein kinase found in human tissues and cell lines and can phosphorylate Cdc25C on serine 216 in vitro. C-TAK1 is distinct from the DNA damage checkpoint kinase Chk1 and may regulate interactions between Cdc25C and 14-3-3 in vivo by phosphorylating Cdc25C at serine 216.
Synonyms: Mark3, Sheep anti-Cdc25c associated protein kinase-1, human
Source
Immunogen: Recombinant, full-length His-tagged human C-TAK1 produced in E. Coli.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Antibody detects c-tak1 by Western blot analysis at 1 to 5 µg/ml. Detects c-tak1 in RKO and HCT116 cell lysates. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Recombinant His-tagged C-TAK1 protein
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Peng, C.Y., et al. C-TAK1 protein kinase phosphorylates human Cdc25C on serine 216 and promotes 14-3-3 protein binding. Cell Growth Differ. 1998, 9, 197-208
2. Ogg, S., et al. Purification of a serine kinase that associates with and phosphorylates human Cdc25C on serine 216. J. Biol. Chem. 1994, 269, 30461-30469
3. Busby, E.C., et al. The radiosensitizing agent 7-hydroxystaurosporine (UCN-01) inhibits the DNA damage checkpoint kinase hChk1. Cancer Res. 2000, 60, 2108-2112
Safety Datasheet(s) for this product:
| EA_Sodium Azide |
|
"Western blot analysis using c-tak1 antibody (Cat. No. X1088P) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3)." |
"Western blot analysis using c-tak1 antibody (Cat. No. X1088P) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3)."