Catalogue
Sheep anti Human Guanalyate Kinase (PDZ 4-5) (MAGI)
Catalog number: K110P$319.00
Add To CartIsotype | IgG |
Product Type |
Polyclonal Antibody |
Units | 250 µg |
Host | Sheep |
Species Reactivity |
Human |
Application |
Western Blotting |
Background
Membrane-associated guanylate kinase with inverted orientation (MAGI3) is a novel, 130 kDa guanylate kinase which is part of a family of multi-PDZ domain containing guanylate kinases. These guanylate kinases are localized to epithelial cell tight junctions. MAGI3 and PTEN/MMAC cooperate to modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions the PTEN/MMAC phosphatase to specific subcellular locations that are involved with the regulation of cell proliferation and survival. MAGI3, as well as other proteins in this family, are made up of an N-terminal guanylate kinase domain, followed by a WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, which is then followed by 6 PDZ domains.
Synonyms: Sheep anti-Membrane-associated guanylate cyclase of inverted orientation-3, PSD95 DiscsLarge Z01 domains 4-5
Source
Immunogen: Recombinant protein corresponding to PDZ domains 4-5 of MAGI-3.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Antibody detects MAGI-3 by Western blot at 1-10 µg/ml. Detects a 130 kDa band in cells transfected with full-length MAGI-3 protein. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Wu, Y., et al. Interaction of the tumor suppressor PTEN/MMAC with a PDZ domain of MAGI3, a novel membrane-associated guanylate kinase. J. Biol. Chem. 2000, 275, 21477-21485
Protein Reference(s)
Database Name: UniProt
Accession Number: Q9H2V6
Safety Datasheet(s) for this product:
EA_Sodium Azide |
"Western blot analysis using MAGI-3, PDZ 4-5 antibody on cell lysates transfected with full-length human MAGI-3 protein." |
"Western blot analysis using MAGI-3, PDZ 4-5 antibody on cell lysates transfected with full-length human MAGI-3 protein."