Catalogue

Background
The Forssman glycolipid is a glycosylceramide possessing a neutral pentasaccharide head group, referred to as the Forssman antigen (Fa) and is a member of the globoseries glycolipid family. Fa has been identified in a number of mammals and exhibits heterogeneity with respect to developmental and cell-type expression among species. Some studies have reported the presence of the Fa in certain Human embryonic and tumor cells.The majority of Human individuals have undetectable levels of Fa. Gastric and colonic tumors from Fa-negative patients, however, contained relatively high levels of Fa. Moreover, compared with normal lung tissue, many lung cancers contained elevated levels of Fa. These observations arouse interest in Fa for a possible role as a Human tumor antigen.

Source
The antibody 33B12 is a Rat anti-Forrsman glycosphingolipid (FGL) antibody derived by fusing Mouse myeloma cells with spleen cells from a Rat immunized with Mouse mammary tumors.

Product
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity
Clone 33B12 and 117C9 detect overlapping epitopes on the Forssman glycolipid hapten (GalNA1- c3aG alNAcD1 -3GalaI-4GalB1-4GlcBl-lCer). Clone 33B12 reacts with the terminal sugar sequence GalNAcd-3GalNAc and is specific for Forssman antigen.

Applications
33B12 is useful for immubohistochemistry on frozen sections. Optimal antibody dilution should be determined by titration.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Sonnenberg A, van Balen P, Hengeveld T, Kolvenbag GJ, Van Hoeven RP, Hilgers J., Monoclonal antibodies detecting different epitopes on the Forssman glycolipid hapten. J Immunol. 1986 Aug 15;137(4):1264-9

Protein Reference(s)

Database Name: UniProt

Accession Number: Q8VI38

Safety Datasheet(s) for this product: