Catalogue

Background
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFα and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFκB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Source
UZ5 is a Rat monoclonal IgG2b antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Lewis Rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface.

Product
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity
UZ5 recognizes a conformational epitope in the CR1 region in murine E-selectin. UZ5 partially inhibits leukocyte adhesion to murine activated endothelial cells.

Applications
UZ5 is suitable for immunoblotting, ELISA, immunoprecipitation, immunohistochemistry on fozen sections (dilution 1:200) and flow cytometry (dilution 1:200).

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Hallmann, R., Zimmermann, U., Sorokin, L. M., Needham, L., and Von der Mark, K. (1995). Adhesion of leukocytes to the inflammed endothelium, Scand J Rheumatol Suppl 101, 107-9. 2. Hammel, M., Weitz-Schmidt, G., Krause, A., Moll, T., Vestweber, D., Zerwes, H. G., and Hallmann, R. (2001). Species-specific and conserved epitopes on Mouse and Human E-selectin important for leukocyte adhesion, Exp Cell Res 269, 266-74.

Protein Reference(s)

Database Name: UniProt

Accession Number: Q00690

Safety Datasheet(s) for this product: