Catalogue

Background
Thymocyte-1 antigen (Thy-1), also known as CD90, is a glycophosphatidylinositol (GPI)-anchored cell surface glycoprotein that is a member of the immunoglobulin superfamily. Expression of Thy-1, originally identified as a marker of thymocyte differentiation in mice, has been found to vary between species. In rats, unlike mice, Thy-1 is expressed on bone marrow cells but not on peripheral T-cells. In both mice and rats, Thy-1 is abundantly expressed on thymocytes and neuronal cells and to a lesser extent on fibroblasts, myofibroblasts, vascular endothelial cells, mammary glands and other non-lymphoid tissues. In many tissues the level of Thy-1 expression changes during cell differentiation. The exact function of Thy-1 has not been fully determined but studies suggest that Thy-1 may be an important regulator of cell-cell and cell-matrix interactions due to its involvement in a range of biological processes including cell growth and differentiation, cell adhesion and migration, apoptotic signalling and tumour suppression.

Source
Thy-1 antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with purified Thy-1 derived from rat brain.

Product
Each vial contains 100µl polyclonal antiserum containing 0.02% sodium azide.

Formulation: Each vial contains 100µl polyclonal antiserum containing 0.02% sodium azide.

Specificity
Thy-1 antiserum is directed against rat thymocyte-1 antigen (Thy-1) and also reacts with mouse Thy-1.

Applications
Thy-1 is suitable for the detection of rat and mouse Thy-1 by immunohistochemical staining of frozen tissue sections. After drying, the sections can be either left unfixed or fixed with acetone at 4°C for 4 min or 95% ethanol at 4°C for 15 min or 4% formaldehyde in phosphate buffered saline pH 7.4 at 20°C for 15 min. Also isolated cells can be immunocytochemically stained by this antiserum. Optimal antibody dilutions for the different applications should be determined by titration. Recommended range 1:50 – 1:100.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Barclay AN, Letarte-Muirhead M, Williams AF. (1975) Purification of the Thy-1 molecule from rat brain. Biochem J. 151:699-706. 2. Letarte-Muirhead M, Barclay AN, Williams AF. (1975) Purification of the Thy-1 molecule, a major cell-surface glycoprotein of rat thymocytes. Biochem J. 151:685-97. 3. Barclay AN, Hydén H. (1978) Localization of the Thy-1 antigen in rat brain and spinal cord by immunofluorescence. J Neurochem. 31:1375-91. 4. Barclay AN. (1979) Localization of the Thy-1 antigen in the cerebellar cortex of rat brain by immunofluorescence during postnatal development. J Neurochem. 32:1249-57. 5. Barclay AN, Hydén H. (1979) Localization of the Thy-1 antigen by immunofluorescence on neurons isolated from rat brain. J Neurochem. 32:1583-86.

Protein Reference(s)

Database Name: UniProt

Accession Number: UniProt: P01830 (Rat), P01831 (Mouse)

Species Accession: Rat, Mouse

Safety Datasheet(s) for this product: