Catalogue
Rabbit anti Mouse Focal Adhesion Kinase (FAK)
Catalog number: X1215P$319.00
Add To CartIsotype | IgG |
Product Type |
Polyclonal Antibody |
Units | 100 µg |
Host | Rabbit |
Species Reactivity |
Mouse |
Application |
Immunoprecipitation Western Blotting |
Background
Focal Adhesion Kinase (FAK) is a 120 kDa tyrosine kinase which become phosphorylated in response to oncogene- and extracellular-matrix integrin-dependent stimulation. Chicken, mouse and human FAK has been isolated and the deduced amino acid sequences showed identity of over 90%. It is expressed in embryonic stem cells, and ubiquitously throughout development, in all adult tissues examined and in many cell lines. The highest expression of FAK is seen in the brain, testes and in osteoclasts. It is distinct from other non-receptor protein tyrosine kinases in that its catalytic domain is flanked by amino- and carboxy-terminal domains of about 400 amino acids each. Also, it does not contain the Src homology 2 or 3 domains, which mediate protein-protein interaction, nor a myristylation site that anchors proteins to the membrane.
Source
Immunogen: Bacterially expressed fusion protein consisting of the C-terminal 150 amino acids of the mouse FAK
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Detect focal adhesion kinase by IP/Western analysis in BALB/c 3T3 fibroblasts1. Detects a 120 kDa band. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Hanks, S.K., et al. Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin. Proc. Natl. Acad. Sci. USA 1992, 89, 8487-8491
2. Ilic, D., et al. Focal adhesion kinase: at the crossroads of signal transduction. J. Cell Sci. 1997, 110, 401-407
Safety Datasheet(s) for this product:
EA_Sodium Azide |
" Immunoprecipitation of FAK using FAK antibody preimmune serum (1) and affinity-purified antibody (2)." |
" Immunoprecipitation of FAK using FAK antibody preimmune serum (1) and affinity-purified antibody (2)."