Rabbit anti Human Sphingosine 1 Phosphate 3 Receptor (EDG-3) CT
Catalog number: X1589P$508.00
Add To CartProduct Type |
Polyclonal Antibody |
Units | 100 µg |
Host | Rabbit |
Species Reactivity |
Human Mouse |
Application |
Immunoprecipitation Western Blotting |
Background
Endothelial cell differentation gene-3 (EDG3) belongs to a family of G-protein couple receptors whose ligands are lysophospholipids. The ligand for EDG3 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family. They have been implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. The couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG3 is expressed in cardiovascular, leukocyte-containing and other tissues.
Synonyms: Endothelial cell differentiation gene 3 C-terminal; Sphingosine-1-Phosphate Receptor-3 (S1P3)
Source
Rabbits were immunized with a synthetic peptide derived from the C terminal of the EDG3 (S1P3) proteins
Product
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form: Unconjugated
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Antibody can be used for Western blotting (5-10 µg/ml) and Immunoprecipitation (see reference 4). Optimal concentration should be evaluated by serial dilutions. Due to low expression of EDG receptors, we recommend use of Pierce Femto Signal substrate for western blot development.
Functional Analysis: Western Blotting
Positive Control: RH7777 cells transfected with EDG3 (S1P3) protein
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Goetzl EJ and An, S. Diversity of cellular receptors and functions for the lysophospholipid growth factos lysophosphatidic acid and sphingosine 1-phosphate. FASEB J. 1998: 12, 1589-98.
2. An, S., Goetzl, E.J., Lee, H. Signaling mechanisms and molecular characteristics of G protein-coupled receptors for lysophosphatidic acid and sphingosine 1-phosphate. J. Cell Biochem. Suppl. 1998: 30-31, 147-57.
3. Singleton PA, Moreno-Vinasco L, Sammani S, Wanderling SL, Moss J, Garcia JG. Attenuation of vascular permeability by methylnaltrexone: role of mOP-R and S1P3 transactivation. Am J Respir Cell Mol Biol. 2007;37(2):222-31.
4. Sun X, Singleton PA, Letsiou E, Zhao J, Belvitch P, Sammani S, Chiang ET, Moreno-Vinasco L, Wade MS, Zhou T, Liu B, Parastatidis I, Thomson L, Ischiropoulos H, Natarajan V, Jacobson JR, Machado RF, Dudek SM, Garcia JG. Sphingosine-1-phosphate receptor-3 is a novel biomarker in acute lung injury. Am J Respir Cell Mol Biol. 2012 Nov;47(5):628-36. doi: 10.1165
Protein Reference(s)
Database Name: SwissProt
Accession Number: Q99500
Species Accession: Human
Safety Datasheet(s) for this product:
EA_Sodium Azide |