Catalogue

Background
Sphingomyelin (SM) is a major component of animal plasma membranes. Its production involves the transfer of phosphocholine from phosphatidylcholine and then onto ceramide. This also yields diacylglycerol as a side product. The reaction is catalysed by SM synthase, an important enzyme in the regulation of diacylglycerol and ceramide as anti- and proapoptotic stimuli. SM synthesis occurs in the lumen of the Golgi as well as on or at the cell surface. It is uncertain which SM enzyme forms are present at these differnet cellular locations. Human, mouse and Caenorhabditis elegans genomes each contain at least two different SM synthase (SMS) genes. Human SMS1 is localised to the Golgi, SMS2 resides primarily at the plasma membrane.

Synonyms: Phosphatidylcholine:ceramide cholinephosphotransferase 2, Sphingomyelin synthase 2

Source

Immunogen: Synthetic peptide derived from the human sphingomyelin synthase 2 protein.

Product

Product Form: Unconjugated

Formulation: Purified IgG in phosphate buffered saline, pH 7.4

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (5-10µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Brain, heart, kidney, liver, muscle and stomach.

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Huitema K, van den Dikkenberg J, Brouwers JF, Holthuis JC Identification of a family of animal sphingomyelin synthases. EMBO J. 2004 Jan 14;23(1):33-44. Epub 2003 Dec 18.

Protein Reference(s)

Database Name: UniProt

Accession Number: Q8NHU3

Species Accession: Human