Catalogue
Rabbit anti Human Sphingomyelin Synthase 1
Catalog number: X2065P$319.00
Add To CartProduct Type |
Antigen Immunoaffinity Purified Polyclonal |
Units | 100 µg |
Host | Rabbit |
Species Reactivity |
Human Mouse |
Application |
ELISA Immunohistochemistry Western Blotting |
Background
A bidirectional lipid cholinephosphotransferases capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and the inverse reaction. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. Directly and specifically recognizes the choline head group on the substrate. Also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. May not function strictly as a SM synthase. Inhibited by bacterial PC-phospholipase C inhibitor D609. Localized to Golgi apparatus; Golgi membrane; multi-pass membrane protein. Possibly present on Brain, heart, kidney, liver, muscle and stomach.
Synonyms: Phosphatidylcholine:ceramide cholinephosphotransferase 1 Transmembrane protein 23, Sphingomyelin synthase 1, Mob protein
Source
Immunogen: Synthetic peptide derived from the human sphingomyelin synthase 1 protein
Product
Product Form: Unconjugated
Formulation: Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08% sodium azide
Purification Method: Antigen Immunoaffinity Purified Polyclonal
Concentration: See vial for concentration
Applications
Antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Brain, heart, kidney, liver, muscle and stomach.
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Huitema K, van den Dikkenberg J, Brouwers JF, Holthuis JC Identification of a family of animal sphingomyelin synthases. EMBO J. 2004 Jan 14;23(1):33-44. Epub 2003 Dec 18.
Protein Reference(s)
Database Name: UniProt
Accession Number: Q86VZ5
Species Accession: Human
Safety Datasheet(s) for this product:
EA_Sodium Azide |
Human kidney section stained with Exalpha’s anti-SMS-1 antibody (Cat X2065P) at 2 ug/ml. Anti-rabbit Ig H+L HRP conjugate (5 ug/ml) without antigen retrieval. |
Human kidney section stained with Exalpha’s anti-SMS-1 antibody (Cat X2065P) at 2 ug/ml. Anti-rabbit Ig H+L HRP conjugate (5 ug/ml) without antigen retrieval.