Catalogue

Background
A bidirectional lipid cholinephosphotransferases capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and the inverse reaction. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. Directly and specifically recognizes the choline head group on the substrate. Also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. May not function strictly as a SM synthase. Inhibited by bacterial PC-phospholipase C inhibitor D609. Localized to Golgi apparatus; Golgi membrane; multi-pass membrane protein. Possibly present on Brain, heart, kidney, liver, muscle and stomach.

Synonyms: Phosphatidylcholine:ceramide cholinephosphotransferase 1 Transmembrane protein 23, Sphingomyelin synthase 1, Mob protein

Source
Rabbits were immunized with a synthetic peptide derived from the mouse sphingomyelin synthase 1 protein

Product
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Product Form: Unconjugated

Purification Method: High Salt Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Brain, heart, kidney, liver, muscle and stomach.

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Huitema K, van den Dikkenberg J, Brouwers JF, Holthuis JC Identification of a family of animal sphingomyelin synthases. EMBO J. 2004 Jan 14;23(1):33-44.

Protein Reference(s)

Database Name: UniProt

Accession Number: Q86VZ5

Species Accession: Human

Safety Datasheet(s) for this product: