Catalogue

Background
SMO is a G protein-coupled receptor (GPCR) that putatively associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. The binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). In basal cell carcinoma there are two somatic mutations SMO, D473-> H473 and R562 ->Q562.

Synonyms: Smoothened homolog [Precursor], SMO, Gx protein, SMO, SMOH

Source

Immunogen: Synthetic peptide derived from the human SMO protein

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1-5µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Brain

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Stone, D.M., et al. The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog.; Nature 384:129-134(1996). 2. Xie, J., et al. Activating Smoothened mutations in sporadic basal-cell carcinoma.; Nature 391:90-92(1998). 3. Liu, Y., et al. 'Fused-Costal2 protein complex regulates Hedgehog-induced Smo phosphorylation and cell-surface accumulation.' Genes Dev. 2007 Aug 1;21(15):1949-63. 4. Moraea, R.C., et al. 'Constitutive activation of smoothened (SMO) in mammary glands of transgenic mice leads to increased proliferation, altered differentiation and ductal dysplasia.' Development. 2007 Mar;134(6):1231-42. 5. Ruiz-Gomez, A., et al. 'The cell biology of Smo signalling and its relationships with GPCRs.' Biochim Biophys Acta. 2007 Apr;1768(4):901-12.

Protein Reference(s)

Database Name: UniProt

Accession Number: Q99835 (Human)

Species Accession: Human

Safety Datasheet(s) for this product: