Catalogue

Background
PP2A is a heterotrimeric phosphatase, ccomposed of A, B and C subunits. The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. Three families of B subunits have been identified thus far. The prototypical B (or PR55) family of subunits is encoded by three genes, B , B and B . The B' (or B56) family consists of at least seven splice variants derived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals. B is a novel isoform of the B class of PP2A regulatory subunits. It is widely expressed in rat tissues and appears to be involved in targeting PP2A to the cytosolic compartment.

Synonyms: pp2A/Bd, rabbit anti protein phosphatase 2A, B subunit delta, PP2A B subunit delta

Source

Immunogen: Synthetic peptide conjugated to KLH derived from the N-terminus of PP2A/B delta

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Detects PP2A/B delta protein in rat testis by Western blot. Detects a 53 kDa band in rat brain, heart, lung, placenta, skeletal muscle, spleen, testis and thymus tissues lysates. Optimal concentration should be evaluated by serial dilutions. Applications: Western Blotting, Immunofluorescence

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Strack, S., et al. Cloning and characterization of Bdelta, a novel regulatory subunit of protein phosphatase 2A FEBS Lett 1999, 460, 462-466

Protein Reference(s)

Database Name: UniProt

Accession Number: Q14738 (Human)Q925E7 (Mouse)P56932 (Rat)

Species Accession: Human, Rat, Mouse

Safety Datasheet(s) for this product: