Catalogue

Background
PHLPP1 is a 1717 aa protein with phosphatase activity that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. May act as a negative regulator of K-Ras signaling in membrane rafts.

Synonyms: KIAA0606, PHLPP, PLEKHE1, SCOP, PHLPP1, PH domain leucine-rich repeat-containing protein phosphatase 1; Pleckstrin homology domain-containing family E member 1; Suprachiasmatic nucleus circadian oscillatory protein

Source

Immunogen: Synthetic peptide derived from the human PHLPP1 protein

Product

Product Form: Pure Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffinity Purification

Concentration: See vial for concentration

Applications
Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Immunohistochemistry

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Gao, T., et al. ‘PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth’ Mol. Cell, 18, 13-24, 2005 2. Bragnard, J., et al. ‘PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms.’ Mol. Cell, 25, 917-931, 2007 3. Gao, T., et al. ‘The phosphatase PHLPP controls the cellular levels of protein kinase C.’ J. Biol. Chem., 283, 6300-6311, 2008

Protein Reference(s)

Database Name: SwissProt

Accession Number: O60346

Species Accession: Human

Safety Datasheet(s) for this product: