Catalogue

Background
PDX1 activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.

Synonyms: Pancreas/Duodenum Homeobox Protein 1; Glucose-sensitive factor; Insulin promoter factor 1; Insulin upstream factor 1;

Source

Immunogen: Synthetic peptide derived from human PDX1 protein.

Product

Product Form: Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

Applications
Antibody can be used for immunohistochemical staining (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Immunohistochemistry

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Stoffers, D.A., et al. ‘Pancreatic agenesis attributable to a single nucleotide deletion in the human IPF1 gene coding sequence.’ Nat. Genet., 15, 106-110 (1997) 2. Macfarlane, M.W., et al. ‘Missense mutations in the insulin promoter factor-1 gene predispose to type 2 diabetes.’ J. Clin. Invest., 104, R33-R39 (1999) 3. Hani, E.H., et al. ‘Defective mutations in the insulin promoter factor-1 (IPF-1) gene in late-onset type 2 diabetes mellitus.’ J. Clin. Invest., 104, R41-R48 (1999) 4. An, R., et al. ‘Pancreatic and duodenal homeobox 1 (PDX1) phosphorylation at serine-269 is HIPK2-dependent and affects PDX1 subnuclear localization.’ Biochem. Biophys Res. Commun., 399, 155-161 (2010)

Protein Reference(s)

Database Name: SwissProt

Accession Number: P52945

Species Accession: Human

Safety Datasheet(s) for this product: