Catalogue
Rabbit anti Human OGR1/GPR68
Catalog number: X1600P$557.00
Add To CartIsotype | IgG |
Product Type |
Polyclonal Antibody |
Units | 100 µg |
Host | Rabbit |
Species Reactivity |
Human |
Application |
Functional Inhibition Immunohistochemistry Western Blotting |
Background
OGR1 (originally identified as GPR68) is a high-affinity receptor for sphingosylphosphorylcholine (SPC). SPC is a member of a class of bioactive lipids which regulate cellular functions. These functions include cell proliferation and growth inhibition, smooth muscle contration and wound healing. SPC also stimulated signalling pathways that induce tyrosine phosphorylation, activate MAP kinases and protein kinase C, modify ion channel activity and increase intracellular Ca2+ concentration. OGR1 is expressed in placenta, lung, liver, spleen, testis, small intestine and peripheral blood leukocytes. It is not found in thymus, ovary, colon, skeletal muscle, kidney or pancreas.
Synonyms: GPR68; Ovarian Cancer G Protein-Coupled Receptor 1
Source
Rabbits were immunized with recombinant protein derived from human OGR1 (GPR68).
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Applications
Antibody can be used for Western blotting (5-10 µg/ml). Western blot should be visualized using a high sensitivity secondary/substrate system (i.e. Pierce’s SuperSignal West Femto Maximum Sensitivity Substrate). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: OGR1 transfected cell lysate
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Xu, Y., et al. 'Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1.' Nature Cell Biol., 2, 261-267 (2000)
2. Singh, L.S., et al. ‘Ovarian cancer G protein-coupled receptor 1, a new metastasis suppressor gene in prostate cancer.’ J. Natl. Cancer Inst., 99, 1313-1327 (2007)
3. Yang, M., et al. ‘Expression of and Role for Ovarian Cancer G-protein-coupled Receptor 1 (OGR1) during Osteoclastogenesis’ J. Biol. Chem., 281, 23598-23605 (2006)
Protein Reference(s)
Database Name: SwissProt
Accession Number: Q15743
Species Accession: Human
Safety Datasheet(s) for this product:
EA_Sodium Azide |
Western blot analysis using OGR1 antibody on cells transfected with OGR1 protein at 10 µg/ml. Blot developed using Pierce’s SuperSignal West Femto Maximum Sensitvity Substrate. |
Western blot analysis using OGR1 antibody on cells transfected with OGR1 protein at 10 µg/ml. Blot developed using Pierce’s SuperSignal West Femto Maximum Sensitvity Substrate.