Catalogue

Background
Acts as a transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis. Efficient DNA binding requires dimerization with another bHLH protein. Expression restricted to the embryonic nervous system.

Synonyms: Class A basic helix-loop-helix protein 6; Neurogenic basic-helix-loop-helix protein; Neurogenic differentiation factor 3; NeuroD3; bHLHa6; NGN-1

Source

Immunogen: Synthetic peptide derived from human neurogenin-1 protein.

Product

Product Form: Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

Applications
Antibody can be used for immunohistochemical staining (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Immunohistochemistry

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Velkey, J.M., et al. ‘Expression of Neurogenin 1 in Mouse Embryonic Stem Cells Directs the Differentiation of Neuronal Precursors and Identifies Unique Patterns of Down-stream Gene Expression.’ Dev. Dyn., 242, 230-253 (2013) 2. Hill, G.W., et al. ‘Netrin-1-mediated axon guidance in mouse embryonic stem cells overexpressing neurogenin-1.’ Stem Cells Dev., 21, 2827-2837 (2012) 3. Choi, C.I., et al. ‘Neural Induction with Neurogenin 1 Enhances the Therapeutic Potential of Mesenchymal Stem Cells in an ALS Mouse Model.’ Cell Transplant., Epub ahead of print (2012)

Protein Reference(s)

Database Name: SwissProt

Accession Number: Q92886

Species Accession: Human

Safety Datasheet(s) for this product: