Catalogue

Background
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Synonyms: H2AFX; H2AX

Source

Immunogen: Synthetic peptide derived from human histone H2a.x protein.

Product

Product Form: Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1-5 µg/ml) and immunohistochemistry (2-5 µg/ml at pH 9.5). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Human bladder, ovary, colon

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Paull, T.T., et al. ‘A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage’ Curr. Biol., 10, 886-895 (2000) 2. Kobayashi, J., et al. ‘NBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain.’ Curr. Biol., 12, 1846-1851 (2002) 3. Stewart, G.S., et al. ‘MDC1 is a mediator of the mammalian DNA damage checkpoint.’ Nature, 421, 961-966 (2003) 4. Lukas, C., et al. ‘Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention.’ EMBO J., 23, 2674-2683 (2004)

Protein Reference(s)

Database Name: SwissProt

Accession Number: P16104

Species Accession: Human

Safety Datasheet(s) for this product: