Catalogue

Background
Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.

Synonyms: Endoribonuclease Dicer; DICER; HERNA

Source

Immunogen: Synthetic peptide derived from human DICER1 protein.

Product

Product Form: Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

Applications
Antibody can be used for immunohistochemical staining (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Immunohistochemistry

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Zhang, H., et al. ‘Single processing center models for human Dicer and bacterial RNase III.’ Cell, 118, 57-68 (2004) 2. Meister, G., et al. ‘Identification of novel argonaute-associated proteins.’ Curr. Biol., 15, 2149-2155 (2005) 3. Haase, A.D., et al. ‘TRBP, a regu1ator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing.’ EMBO Rep., 6, 961-967 (2005) 4. Kok, K.H., et al. ‘Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA.’ J. Biol. Chem., 282, 17649-17657 (2007) 5. Melo, S.A., et al. ‘A TARBP2 mutation in human cancer impairs microRNA processing and DICER1 function.’ Nat. Genet., 41, 365-370 (2009)

Protein Reference(s)

Database Name: SwissProt

Accession Number: Q9UPY3

Species Accession: Human

Safety Datasheet(s) for this product: