Catalogue

Background
DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells (1). This enzyme is specific to germ cells and is necessary for germ cell development (2). Mouse DDX4 was shown to interact with Dicer, suggesting a role in microRNA-mediated RNA silencing (1).

Synonyms: Probable ATP-dependent RNA Helicase DDX4; MVH; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4

Source

Immunogen: Synthetic peptide derived from the human DDX4 protein

Product

Product Form: Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

Applications
Antibody can be used for immunohistochemistry (2-5 µg/ml at pH 6.2) and Western blot (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Human testis tissue

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Kotaja, N., et al. ‘The chromatoid body of male germ cells: similarity with processing bodies and presence of Dicer and microRNA pathway components.’ Proc. Natl. Acad. Sci., 103, 2647-2652 (2006) 2. Albamonte, M.S., et al. ‘The developing human ovary: immunohistochemical analysis of germ-cell-specific VASA protein, BCL-2/BAX expression balance and apoptosis.’ Hum. Reprod. 23, 1895-1901 (2008)

Protein Reference(s)

Database Name: SwissProt

Accession Number: Q9NQI0

Species Accession: Human

Safety Datasheet(s) for this product: