Catalogue

Background
Voltage-sensitive calcium channels (VSCCs) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1b gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (hva) group and are blocked by omega-conotoxin-GVIA (omega-ctx-GVIA) and by omega-agatoxin-IIIA (omega-aga-IIIA). They are however insensitive to dihydropyridines (dhp), and omega-agatoxin-IVA (omega- aga-IVA). Calcium channels containing alpha-1b subunit may play a role in directed migration of immature neurons.

Synonyms: Voltage-gated calcium channel subunit alpha Cav2.2, Calcium channel, L type, alpha-1 polypeptide isoform 5, Brain calcium channel III

Source

Immunogen: Synthetic peptide derived from the rat alpha1B calcium channel conjugated to KLH

Product

Product Form: Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1:400 dilution). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Isoform Alpha-1b-1 and isoform Alpha-1b-2 are expressed in the central nervous system, but not in skeletal muscle or aorta.

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Dubel, S.J. et.al. 'Molecular cloning of the alpha-1 subunit of an omega-conotoxin-sensitive calcium channel' Proc. Natl. Acad. Sci. U.S.A. 89 (11), 5058-5062 (1992) 2. Snutch,T.P.et.al. 'Rat brain expresses a heterogeneous family of calcium channels' Proc. Natl. Acad. Sci. U.S.A. 87 (9), 3391-3395 (1990) 3. Hell, J.W. et.al. 'Differential phosphorylation of two size forms of the N-type calcium channel alpha 1 subunit which have different COOH termini' J. Biol. Chem. 269 (10), 7390-7396 (1994) 4. Sunan, A.A. et.al. 'The C terminus of the Ca channel alpha1B subunit mediates selective inhibition by G-protein-coupled receptors.' J Neurosci. 21(19), 7587-97. (2001)

Protein Reference(s)

Database Name: UniProt

Accession Number: Q00975

Species Accession: Human

Safety Datasheet(s) for this product: