Catalogue

Background
Voltage-sensitive calcium channels (VSCCs) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-ctx-GVIA) and omega-agatoxin-IVA (omega-aga-IVA). The overall conclusion is that deletion of the beta 3 subunit affects at least three distinct types of High voltage activated Ca2+ channel (L,N and P/Q types), but no single type of channel is solely dependent on beta 3.

Synonyms: Voltage-Dependent L-Type Calcium Channel, beta3 subunit

Source

Immunogen: Synthetic peptide derived from the rat beta3 calcium channel conjugated to KLH

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
This antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Rat brain lysate

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Castellano,A.et.al., Cloning and expression of a third calcium channel beta subunit J. Biol. Chem. 268 (5), 3450-3455 (1993 2. Castellano,Aet.al. Cloning and expression of a neuronal calcium channel beta subunit J. Biol. Chem. 268 (17), 12359-12366 (1993) 3. Smith SM, et al.Neuronal voltage-activated calcium channels: on the roles of the alpha 1E and beta 3 subunits.Ann N Y Acad Sci USA 868, 175-198 (1999)

Protein Reference(s)

Database Name: UniProt

Accession Number: P54284

Species Accession: Human

Safety Datasheet(s) for this product: