Catalogue
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Rabbit anti Human Autotaxin
Catalog number: X1653P$319.00
Add To CartIsotype | IgG |
Product Type |
Polyclonal Antibody |
Units | 100 µg |
Host | Rabbit |
Species Reactivity |
Human |
Application |
Enzyme Immunoassay Western Blotting |
Background
Autotaxin (ATX), a tumor cell motility- stimulating protein is an ecto/exo-enzyme with significant homology to the plasma cell membrane differentiation antigen PC-1. ATX is a 99kDa core protein and may run as a 125-kDa glycoprotein when isolated from the human melanoma cell line (A2058). ATX, like PC-1 can hydrolyze the type I phosphodiesterase substrate p-nitrophenyl thymidine-5'-monophosphate. Autotaxin has a novel motility- regulating function for this class of ecto/exo-enzymes.
Synonyms: EctonucleotAutotaxin (ATX), a tumor cell motility- stimulating protein is an ecto/exo-enzyme with significant homology to the plasma cell membrane differentiation antigen PC-1. ATX is a 99kDa core protein and may run as a 125-kDa glycoprotein when isolate
Source
Immunogen: Synthetic peptide derived from the autotaxin protein
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Applications
Antibody can be used for Western blotting (5-10 µg/ml) and EIA. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Human brain lysate (Cat. No. X1633C)
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
[1] Murata J., Lee H.Y., Clair T., Krutzsch H.C., Arestad A.A., Sobel M.E., Liotta L.A., Stracke M.L.; cDNA cloning of the human tumor motility-stimulating protein, autotaxin, reveals a homology with phosphodiesterases.; J. Biol. Chem. 269:30479-30484(1994).
[2] Lee H.Y., Murata J., Clair T., Polymeropoulos M.H., Torres R., Manrow R.E., Liotta L.A., Stracke M.L.; Cloning, chromosomal localization, and tissue expression of autotaxin from human teratocarcinoma cells.; Biochem. Biophys. Res. Commun. 218:714-719(1996).
[3] Kawagoe H., Soma O., Goji J., Nishimura N., Narita M., Inazawa J., Nakamura H., Sano K.;
Molecular cloning and chromosomal assignment of the human brain-type phosphodiesterase I/nucleotide.; Genomics 30:380-384(1995).
[4] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
Protein Reference(s)
Database Name: SwissProt
Accession Number: Q13822
Species Accession: Human
Safety Datasheet(s) for this product:
EA_Sodium Azide |
!["
Western blot analysis using autotaxin antibody on human brain lysate."](https://exalpha-7d62.kxcdn.com/ProductImages/X1653P\X1653P-Figure01.jpg)
" Western blot analysis using autotaxin antibody on human brain lysate."