Catalogue
Rabbit anti Human Acid Ceramidase 1
Catalog number: X2668P$330.00
Add To CartIsotype | IgG |
Product Type |
Antigen Immunoaffinity Purified Polyclonal |
Units | 100 µg |
Host | Rabbit |
Species Reactivity |
Human Mouse Rat |
Application |
ELISA Immunohistochemistry Western Blotting |
Background
Acid ceramidase is a lipid hydrolyase responsible for the degradation of ceramide into sphingosine and free fatty acids within lysosomes. It can also synthesize ceramide from sphingosine and free fatty acids as well. The reverse activity is pH dependent (6.0 vs 4.5, respectively). This suggests that the enzyme may have diverse functions within cells dependent on its subcellular location and the local pH. Recent studies have shown that acid ceramidase activity is aberrantly expressed in several human cancers and that it might be a useful drug target. As an example, inhibitors of enzyme activity have been shown to slow the growth of cancer cells alone or in combination with other established, anti-oncogenic treatments. Aberrant activity has also been shown in AlzheimerÕs disease and overexpression may prevent insulin resistant (Type II) diabetes by free fatty acids.
Synonyms: N-acylsphingosine amidohydrolase (Acid ceramidase) 1; ASAH1
Source
Immunogen: Synthetic peptide derived from the C terminal region of the ASAH1 protein.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Antigen Immunoaffiinity Purification
Concentration: See vial for concentration
Applications
Antibody can be used for Western blotting (1-5 µg/ml) and Immunohistochemistry on paraffin-embedded tissues (10 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Human heart tissue
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Park, J.H., et al. 'Acid ceramidase and human disease.' Biochim Biophys Acta 2006; 1758:2133-2138.
2. Zeidan, Y.H., et al. 'Acid ceramidase but not acid sphingomyelinase is required for tumor necrosis factor-{alpha}-induced PGE2 production.' J. Biol. Chem. 2006; 281:24695-24703.
3. Shtraizent, N., et al. 'Autoproteolytic cleavage and activation of human acid ceramidase.' J. Biol. Chem. 2008; 283:11253-11259.
Protein Reference(s)
Database Name: UniProt
Accession Number: Q53H01-HumanQ9EQJ6-RatQ9WV54-Mouse
Species Accession: Human, Rat, Mouse
Safety Datasheet(s) for this product:
EA_Sodium Azide |
Immunohistochemical staining of human heart tissue using Acid Ceramidase 1 antibody at 10 µg/ml.