Catalogue

Background
Receptor-like protein tyrosine phosphatases (RPTPs) represent a new family of transmembrane proteins that are thought to transduce external signals by dephosphorylating phosphotyrosine residues on intracellular substRates. RPTPu is a prototypic receptor-like protein-tyrosine phosphatase (RPTP) that mediates homotypic cell-cell interactions. Intracellularly, RPTPu consists of a relatively large juxtamembrane region (about 150 amino acids) and two conserved phosphatase domains. As in most RPTPs, the first (membrane-proximal) PTP domain of RPTPu is Catalytically active, whereas its second (C-terminal) domain is inactive. RPTPu is involved in cell-cell adhesion through homophilic interactions and may also play a key role in signal transduction and growth control.

Source
3D7 is a Mouse monoclonal IgG2a antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with ExG protein, secreted by NIH-3T3 transfected cells and purified with glutahione beads. The cells have been transfected using a fusion construct, termed ExG, encoding the entire ectodomain of the Human RPTPu molecule (aa 1-742), fused COOH terminally to GST.

Product
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity
Monoclonal antibodies 3G4 and 3D7 have been directed anti the extracellular domain of RPTPu.

Applications
3D7 is useful for western blot, immunofluorescence, immunoprecipitation and flow cytometry. Optimal antibody dilution should be determined by titration.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Gerben C. M. Zondag, Albert B. Reynolds§, and Wouter H. Moolenaar. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 275, No. 15, Issue of April 14, pp. 11264–11269, 2000.

Safety Datasheet(s) for this product: