Catalogue

Background
Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A (SP-A) to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis.

Source
Mouse monoclonal antibody 2A12, obtained after immunization of mice with 3-Nitrotyrosine-KLH

Immunogen: 3-Nitrotyrosine-KLH

Product
100 µg monoclonal antibody in 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, 3mM sodium azide, pH 7.5

Product Form: Unconjugated

Purification Method: Prot A/G chromatography

Concentration: See vial for concentration

Specificity
Nitrotyrosine

Applications
Antibody can be used for Western blotting (Suggested dilution - 1:1000) and immunohistochemistry. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Knight-Lozano, C.A., et al. 'Cigarette smoke exposure and hypercholesterolemia incrase mitochondrial damage in cardiovascular tissues.' Circulation, 105, 849-854 (2002) 2. Khan, F. & Siddiqui, A.A. 'Prevalence of anti-3-nitrotyrosine antibodies in the joint synovial fluid of patients with rheumatoid arthritis, osteoarthritis and systemic lupus erythematosus.' Clin Chim Acta, 370, 100-107 (2006). 3. Deeb, R.S., et al. 'Tyrosine nitration in prostaglandin H2 synthase.’ J Lipid Res, 43, 1718-1726 (2002). 4. Blanchard-Fillion, B., et al. 'Metabolism of 3-nitrotyrosine induces apoptotic death in dopaminergic cells.' J Neurosci, 26, 6124-6130 (2006).

Safety Datasheet(s) for this product: