Catalogue

Background
CD43 antigen is expressed on all T cells, NK cells, myeloid cells and monocytes as well as on CD5 positive mature B cells. It is also present on all immature hematopoietic cells in the bone marrow. The antigen is deficient in patients with Wiskott-Aldrich syndrome. A soluble form of CD43 is present in Human Serum.

Source
MT1 is a monoclonal antibody derived by fusion of X63 Mouse myeloma cells with spleen cells from of Balb/c mice, intraperitoneally immunized with Human monocytes isolated by centrifugal elutriation. Immunizations were carried out at least twice at 2-week intervals with: A) cell suspensions of a lymph node involved by Hodgkin's disease; B) cell suspensions of a lymph node involved by Bchronic lymphatic leukemia; C) cells of Hodgkin's cell line DEV, respectively. No adjuvants were used (3).

Product
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity
Clone MT1 produces Mouse IgG1 immunoglobulins reactive with a 95-115 kD highly sialated glycoprotein. This antibody is used to identify B cell lines and myeloma cells. It may be used in the diagnosis of chronic lymphocytic leukemias (CLL), as an alternative to CD5. The antigen detected by MT1 is the only marker expressed on neoplasms of the very early precursor cells. In immunohistochemistry it reacts with T cells, macrophages, myeloid cells and B cells (weak). It is used for the typing of lymphomas in paraffin sections. CD43 may function as an adhesion molecule via interaction with CD54 although this has not been definitely established. It may also inhibit leucocyte interactions with other cells. The antigen is involved in the activation of T-cells, B cells, NK cells and monocytes. The membrane proximal portion of the cytoplasmic domain mediates an association with the cytoskeleton.

Applications
The antibody is suitable for flow cytometry for analysis of blood and bone marrow samples or in immunohistochemistry using cytospots, paraffin or frozen tissue sections. Optimal antibody dilution should be determined by titration.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Barclay, A., et al. (1997). The Leucocyte antigen factsbook. Academic Press. London. 2. Schmid, K., Hediger M.A., Brossmer, R., Collins, J.H., Haupt, H. Marti, T., Offner, G.D., Schaller, J., Takaqaki, K., Walsh, M.T., et al. (1992). Amino acid sequence of Human plasma galactoglycoprotein: identity with the extracellular region of CD43 (sialophorin). Proc Natl Acad Sci U S A 89, 663-7. 3. Poppema, S., Hollema, H., Visser, L. and Vos, H. (1987). Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections. Am J Pathol. 127, 418-29. 4. Poppema, S. and Visser, L. (1987). Biotest bulletin 31, 418-29. Knapp, W., et al. eds. (1989). Leucocyte typing workshop IV. Oxford university press. 5. Manjunath, N., Correa, M., Ardman, M. and Ardman, B. (1995). Negative regulation of T-cell adhesion and activation by CD43. Nature 377, 535-8.

Safety Datasheet(s) for this product: