Catalogue
Mouse anti Human p34 cdc-2 nuclear protein
Catalog number: X1236M$319.00
Add To CartClone | POH-1 |
Isotype | IgG2a |
Product Type |
Monoclonal Antibody |
Units | 100 µg |
Host | Mouse |
Species Reactivity |
Bovine Human Mouse Rat Xenopus |
Application |
Immunocytochemistry Immunohistochemistry (paraffin) Immunoprecipitation Western Blotting |
Background
p34(Cdc2) kinase is a an essential nuclear protein for mitosis in human cells. During the prophase/metaphase transition the inactive phosphorylated form of p34(cdc2) is dephosphorylated to make the active form of the protein. This dephosphorylation is performed by the p80(cdc25) dual-specificity phosphatase. The regulatory subunit of p34(cdc2) is cyclin B, a 47-52 kDa protein. The p34(cdc2) protein is the catalytic subunit of the M phase-promoting factor (MPF) which is intracellular factor responsible for entry into M phase. Three mechanisms are responsible for the activitation of MPF at the onset of mitosis: binding of p34(cdc2) to cyclin B, a series of phosphorylation/dephosphorylation events on the two subunits and translocation of the complex to the nucleus or microtubules.
Source
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant human p34.cdc2 fusion protein and mouse myeloma cells.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Applications
Detects three bands within the 34 kDa region corresponding to p34 and its cleavage products. Can also be used for IHC on paraffin-embedded tissue (10 µg/ml) as well as immunocytochemistry and immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: HeLa cell lysate
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Lukas, J., et al. Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur. J. Biochem. 1992, 207, 169-176
2. Borgne, A. & Meijer, L. Sequential dephosphorylation of p34(cdc2) on Thr-14 and Tyr-15 at the prophase/metaphase transition. J. Biol. Chem. 1996, 271, 27847-27854
3. Kao, G.D., et al. p34(Cdc2) kinase activity is excluded from the nucleus during the radiation-induced G2 arrest in HeLa cells. J. Biol. Chem. 1999, 274, 34779-34784
Protein Reference(s)
Database Name: SwissProt
Accession Number: P06493
Species Accession: Human
Safety Datasheet(s) for this product:
EA_Sodium Azide |