Catalogue

Background
Muc1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed in >90% breast carcinomas and metastases. In normal mammary gland it is expressed in apical surface of glandular epithelium. In breast cancer Muc1 is overexpressed; is underglycosylated and the apical localization is lost. Muc1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400kDa) and a smaller subunit (14-28kDa) noncovalently associated with each other. Transgenic Muc1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands1. Muc1 can act as a ligand for ICAM-1 on HUVEC cells; it can bind -catenin, GSK3 and it associates with Grb2-SOS upon phosphorylation.

Synonyms: Episialin

Source
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a Muc1 60mer tandem repeat NH2-(VTSAPDTRPAPGSTAPPAHG)3-COOH and mouse myeloma SP2 cells.

Product
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Product Form: Unconjugated

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Antibody can be used for immunohistochemistry on frozen and paraffin embedded tissues, ELISA and Western blotting. Optimal concentration should be evaluated by serial dilutions. Antibody can only detect Muc1 if the threonine residue of the PDTRPAP of the tandem repeat region is non-glycosylated4.

Functional Analysis: Western Blotting

Positive Control: MCF-7 and T47D cells

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Mommers E C, et al. 'Abberant expression of MUC1 mucin in ductal hyperplasia and ductal carcinoma In situ of the breast.' Int J Cancer 1999: 84:466-469. 2. Schol, D.J. et al., Epitope fingerprinting using overlapping 20-mer peptides of the MUC 1 tandem repeat sequence. Tumor Biol. Suppl. 1998: 19, 35-45. 3. Ryuko K et al. 'Characterization of a new MUC1 monoclonal antibody (VU-2-G7) directed to the glycosylated PDTR sequence of MUC1.' Tumor Biology 2000: 21, 197-210. 4. ten Berge, R.L., et al. 'MUC1 (EMA) is preferentially expressed by ALK positive anaplastic large cell lymphoma, in the normally glycosylated or only partly hypoglycosylated form.' J. Clin. Pathol. 2001, 54, 933-939. 5. Treon, S.P., et al. 'Muc-1 Core Protein Is Expressed on Multiple Myeloma Cells and Is Induced by Dexamethasone.' Blood 1999, 93, 1287-1298.

Protein Reference(s)

Database Name: SwissProt

Accession Number: P15941

Species Accession: Human

Safety Datasheet(s) for this product: