Catalogue
Mouse anti Human Keratin 18
Catalog number: X1254M$319.00
Add To CartClone | DC10 |
Isotype | IgG1 |
Product Type |
Monoclonal Antibody |
Units | 100 µg |
Host | Mouse |
Species Reactivity |
Human |
Application |
Immunohistochemistry (frozen & paraffin) Western Blotting |
Background
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.CK18 is an acidic keratin which is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. CK18 exists in combination with CK8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only cytokeratins 8 and 18.
Source
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with human PMC-42 breat carcinoma cells and mouse myeloma cells.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Applications
Antibody detects human keratin 18 by Western blot at 45 kDa. Also detects CK18 by immunohistochemistry on frozen and paraffin-embedded tissues. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Lauerova, L., et al. 'Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule.' Hybridoma 1988, 7, 495-504
2. Moll, R., et al. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982, 31, 11-24
Protein Reference(s)
Database Name: SwissProt
Accession Number: P05783
Species Accession: Human
Safety Datasheet(s) for this product:
EA_Sodium Azide |