Catalogue
Mouse anti Human Insulin
Catalog number: X1843M$319.00
Add To CartClone | E2E3 |
Isotype | IgG1 |
Product Type |
Monoclonal Antibody |
Units | 100 µg |
Host | Mouse |
Species Reactivity |
Bovine Human Porcine |
Application |
ELISA Immunohistochemistry Western Blotting |
Background
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.
Source
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full-length insulin protein and mouse myeloma cells.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Applications
Antibody can be used for Western blotting (1-5 µg/ml), immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-2 µg/ml, incubate at room temp. for at least 1 hr.) and ELISA. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Roth, J., et al. 'Distribution patterns of proinsulin and insulin in human insulinomas: an immunohistochemical analysis in 76 tumors.' Virchows Arch B Cell Pathol Incl Mol Pathol 63, 51-61 (1992).
2. Nykiforuk, C.L., et al. 'Transgenic expression and recovery of biologically active recombinant human insulin from Arabidopsis thaliana seeds.' Plant Biotechnology J. 4, 77-85 (2006)
3. Lee, A.H., et al. 'XBP-1 is required for biogenesis of cellular secretory machinery of exocrine glands.' EMBO J., 24, 4368-4380 (2005)
Protein Reference(s)
Database Name: UniProt
Accession Number: P01308
Species Accession: Human
Safety Datasheet(s) for this product:
EA_Sodium Azide |