Catalogue

Background
CD62L is the equivalent of the mouse MEL-14 with a molecular weight of 80 kDa. CD62L is on 60-75% of peripheral blood lymphocytes, Approximately 70-80% of T cells, 10% of thymocytes and most B cells. CD62L reacts with monocytes, granulocytes and a subset of NK cells. The selectins represent a family of adhesion molecules, expressed by leukocytes and endothelial cells that are involved in the regulation of leukocyte traffic. Human leukocyte L selectin is also called LAM-1, LECAM-1, or gp90MEL-14. This antibody to L selectin blocks greater than 95% of lymphocyte adhesion to peripheral lymph node high endothelial venules.

Synonyms: CD62L PE

Source

Immunogen: CD62L=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with perherial blood T lymphocytes.

Product

Product Form: RPE

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Purification Method: Protein A/G Chromatography

Concentration: Titered for flow cytometry

Applications
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at 4-8°C. DO NOT FREEZE

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Identification of a specific glycoprotein ligand for P-selectin (CD62) on myeloid cells. J Cell Biol 1992 Jul;118(2):445-56. Moore KL; Stults NL; Diaz S; Smith DF; Cummings RD; Varki A; McEver RP 2. Cytoplasmic domain of P-selectin (CD62) contains the signal for sorting into the regulated secretory pathway. Mol Biol Cell 1992 Mar;3(3):309-21. Disdier M; Morrissey JH; Fugate RD; Bainton DF; McEver RP 3. Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins. Cell 1991 May 31;65(5):859-73. Lawrence MB; Springer TA 4. Biochemical and immunohistochemical characteristics of CD62 and CD63 monoclonal antibodies. Expression of GMP-140 and LIMP-CD63 (CD63 antigen) in human lymphoid tissues. Virchows Arch B Cell Pathol 1991;61(4):269-77. Metzelaar MJ; Schuurman HJ; Heijnen HF; Sixma JJ; Nieuwenhuis HK 5. CD62/P-selection recognition of myeloid and tumor cell sulfatides. Cell 1991 Oct 4;67(1):35-44. Aruffo A; Kolanus W; Walz G; Fredman P; Seed B 6. Structure and chromosomal location of the gene for endothelial-leukocyte adhesion molecule 1. J Biol Chem 1991 Feb 5;266(4):2466-73. Collins T; Williams A; Johnston GI; Kim J; Eddy R; Shows T; Gimbrone MA Jr; Bevilacqua MP

Protein Reference(s)

Database Name: UniProt

Accession Number: P14151

Safety Datasheet(s) for this product: