Catalogue

Background
CD61 recognizes an Mr 110-kdalton (kDa) protein, also known as gpIIIa, the common §subunit (integrin §3-chain) of the gpIIb/IIIa complex and the vitronectin receptor (VNR). The gpIIb/IIIa complex and the VNR are integrins, ie, a/b-heterodimeric glycoprotein complexes that are involved in cell adhesion. With the CD41 antigen (gpIIb or aIIb), the CD61 antigen forms the gpIIb/IIIa complex, which acts as a receptor for fibrinogen, von Willebrand factor (vWf), fibronectin and vitronectin on activated platelets. With the CD51 antigen (VNR a-chain or av), the CD61 antigen forms the VNR, which mediates activation-independent cell adhesion to vitronectin, vWf, fibrinogen, and thrombospondin. The CD61 antigen is found on all normal resting and activated platelets. The CD61 antigen is also found on endothelial cells, megakaryocytes, on some myeloid, erythroid and T-lymphoid leukemic cell lines.

Synonyms: CD61FITC

Source

Immunogen: CD61=Derived from the hybridization of mouse X63.Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with purified platelet membrane glycoproteins.

Product

Product Form: FITC

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Purification Method: Protein A/G Chromatography

Concentration: Titered for flow cytometry

Applications
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at 4-8°C. DO NOT FREEZE

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. von dem Borne AEGKr, Modderman PW, Admiraal LG, Nieuwenhuis KH. Platelet antibodies, the overall results. In: Knapp W,Dorken B, Gilks WR, et al, eds. Leucocyte Typing IV, White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:950-966. 2. Modderman PW. Cluster report: CD61. In: Knapp W, Dorken B, Gilks WR, et al, eds. Leucocyte Typing IV, White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:1025. 3. Modderman PW. New clusters: CD29/CDw49, CD47, CD51, CD55, and CD61. In: Knapp W, Dorken B, Gilks WR, et al, eds. Leucocyte Typing IV, White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:1017-1019. 4. Springer TA. Adhesion receptors of the immune system. Nature. 1990;346:425-433. 5. Parmentier S, Kaplan C, Catimel B, McGregor JL. New families of adhesion molecules play a vital role in platelet functions. Immunol Today. 1990;11:225-227. 6. Fijnheer R, Modderman PW, Veldman H, et al. Detection of platelet activation with monoclonal antibodies and flow cytometry. Transfusion. 1990;30:20-25. 7. von dem Borne AEGKr, Modderman PW. Cluster report: CD41. In: Knapp W, Dorken B, Gilks WR, et al, eds. Leucocyte Typing IV, White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:997-999. 8. Jennings LK, Ashmun RA, Wang WF, Dockter ME. Analysis of human platelet glycoproteins IIb-IIIa and Glanzmann's thrombasthenia in whole blood by flow cytometry. Blood. 1986;68:173-179.

Protein Reference(s)

Database Name: UniProt

Accession Number: P05106

Safety Datasheet(s) for this product: