Catalogue

Background
CD54, M.W. 90,000, antigen is the Intercellular Adhesion Molecule-1 (ICAM-1) and is the ligand for LFA-1 mediated adhesion. CD54 is weakly expressed on peripheral blood leukocytes. Upon activation the antigen is expressed strongly on B cells, T cells, macrophages and granulocytes. ICAM-1 is also inducible on endothelial cells, epithelial cells and fibroblasts.

Synonyms: CD54

Source

Immunogen: CD54=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with the major histocompatibility complex (MHC) class II-negative human b-lymphoblastoid cell line 6.16c13

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20° C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. ICAM-1 a ligand for LFA-1 dependent adhesion of B,T and myeloid cells. Makgoba, M.W., Sanders, M.E., Ginther, Luce, G.E., Dustin, M.L., Springer,T.A., Clark, E.A., Mannoni, P., Shaw, S., Nature, 1988:331:86. 2. Supergene families meet in the immune system. Dustin, M.L., Staunton, D.E., and Springer, T.A., Immunol. Today. 1988:9:213. 3. Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1. Staunto, D.E., Dustin, M.L., and Springer, T.A., Nature. 1989:339:61. 4. Leukocytes adhesion to cells. Molecular basis, Physiological Relevance and Abnormalities. Patarroyo, M., and Makgoba, M.W., Scand. J. Immunol. 1989;30:129. 5. A soluble form of intercellular adhesion molecule-1 inhibits rhinovirus infection. Marlin SD, Staunton DE, Springer TA, Stratowa C, Sommergruber, W., and Merluzzi, V.J., Nature. 1990;44:70.

Protein Reference(s)

Database Name: UniProt

Accession Number: P05362

Safety Datasheet(s) for this product: