Catalogue

Background
The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is present on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells. Identification of human T cells expressing the 22-28,000 M.W. surface antigen. Identification of CD4 human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes.

Synonyms: CD3 FITC - CD4 PE

Source

Immunogen: CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes. CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.

Product

Product Form: Bi-Test (FITC/RPE) Reagent

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Purification Method: Protein A/G Chromatography

Concentration: Titered for flow cytometry

Applications
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at 4-8°C. DO NOT FREEZE

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Thymus Dependent Membrane Antigens in Man: Inhibition of Cell-Mediated Lympholysis by Monoclonal Antibodies to the TH-2 Antigen. Evans,R.L., Wall,D.W., Platsoucas,C.D., Siegal,F.P., Fikrig,S.M., Testa,C.M, and Good,R.A. Proc. Nat. Acad. Sci. 78,544,1981 2. Novel Immunoregulatory Functions of Phenotypically Distinct Subpopulations of CD4+ cells in the Human Neonate. Clement,L.T., Vink,P.E., Bradley,G.E. J. Immunology 145(1):102-8,1990 3. Antigen Presentation by the CD4 Positive Monocyte Subset. Szabo,G., Miller,C.L., Kodys,K., J. Leukoc. Biol. 47(2): 111-20,1990 4. Human Immunodeficiency Virus Infection is Efficiently Mediated by a Glycolipid-Anchored form of CD4. Diamond,D.C., Finberg,R., Chaudhuri,S., Sleckman,B.P., Burakoff,S.J., Proc. Natl. Acad. Sci. 87(13):5001-5,1990 5. Development Regulation of the Intrathymic T cell Precursor Population. Adkins,B., J. Immunol. 146(5):1387-93,1991 6. Knowles RW. Immunochemical analysis of the T cell-specific antigens. In : Reinhert EL, Haynes BF, Nadl LM and Bernstein ID. eds. Leukocyte Typing II, Hunan T Lymphocytes. New York, NY: Springer-Verlag; 1986:259 7. Kurrle R. Cluster Report:CD3. In:Knapp W, Dorken B, Gilks WR, Reiber EP, Schmidt RE, Stein H, and von dem Borne AEG Kr, eds. Leukocyte Typing IV, White cell Differentation Antigens. Oxford, England: Oxford Press 1989:293 8. Signal transduction via CD4,CD8 and CD28 in mature and immature thymocytes. Implications for thymic selection. Turka LA, Linsley PS, Paine R 3d, Schieven Gl, Thompson GB, Ledbetter JA, J. Immunol. 1991 Mar :146(5): 1428-36 9. T cell receptor/CD3-signaling induces death by apoptosis in human T cell receptor gamma delta + Tcells. Janssen O, Wesselborg S, Heckl-Ostreicher B, Pechhold K, Bender A, Schondelmaier S, Moldenhauer G, Kabelitz D I Immunol. 1991 Jan146(1):35-9 10. Clonal analysis of human CD4-CD8-CD3- thymocytes highly purified from postnatal thymus Hori T, Spits H J. Immunol. 1991 Apr 146(7):2116-21 11. Molecular cloning of the CD3 zeta subunit identifies a CD3 zeta-related product in thymus-derived cells Jin YJ, Claton LK, Howard FD, Koyasu S, Sieh M, Steinbrich R, Tarr GE, Reinherz EL. Proc Natl Acad Sci usa 1990 Ma: 87(9):3319-2

Protein Reference(s)

Database Name: UniProt

Accession Number: P07766, P01730

Safety Datasheet(s) for this product: