Catalogue
Mouse anti Human CD23
Catalog number: 0231$185.00
Add To CartClone | LVNC-4 |
Isotype | IgG1 |
Product Type |
Single-Color Reagent |
Units | 100 µg |
Host | Mouse |
Species Reactivity |
Human |
Application |
Flow Cytometry |
Background
CD23 antigen is an integral membrane glycoprotein, Mr. 45 kdaltons The CD23 antigen is present on approximately all pre-B lymphocytes, plasma cells CD25 positive Ki-1 cells and HLA-DR. CD23 antigen is also expressed on Lymphoma, T Cell Lymphomas and B-CLLs. CD23 antigen is present on HodgkinÕs and Reed Sternberg cells).
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Applications
PBMC: Add10 µl of MAB/106 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Functional Analysis: Flow Cytometry Staining
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Room Temperature
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. CD23: a multi-lineage cell activation molecule with a split personality. Int J Clin Lab Res 1992; 22(2):73-80Malavasi F; Funaro A; Alessio M; DeMonte LB; Ausiello CM; Dianzani U; Lanza F; Magrini E; Momo M; Roggero S
2. Eleven lymphoid phenotypic markers in HIV infection: selective changes induced by zidovudine treatment. J Acquir Immune Defic Syndr 1992;5(9):890-7Bass HZ; Hardy WD; Mitsuyasu RT; Wang YX; Cumberland W; Fahey JL
3. Three-color cytofluorometric analysis of CD8 cell subsets in HIV-1 infection. J Acquir Immune Defic Syndr 1991;4(12):1227-32 Prince HE; Jensen ER
Protein Reference(s)
Database Name: UniProt
Accession Number: P06734
Safety Datasheet(s) for this product:
EA_Sodium Azide |