Catalogue

Background
(Mac-1), CD11b/CD18 complex is a leukocyte adhesion receptor to intercellular adhesion molecules (ICAM's). CR3 alpha chain (M.W. 165,000) is on virtually all peripheral blood neutrophils, eosinophils, monocytes and approximately 30% of peripheral blood lymphocytes.

Synonyms: CD11b FITC

Product

Product Form: FITC

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Purification Method: Protein A/G Chromatography

Concentration: Titered for flow cytometry

Applications
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at 4-8°C. DO NOT FREEZE

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1.Structure and function of the leukocyte adhesion molecules CD11/CD18. Arnaout, M.A., Blood . 1990 Mar; 75(5):1037-50. 2. Leukocyte adhesion to endothelial cells. Wautier, ,J.L., Sediadi, H., Vilette, d., Weill, D., Wautier, M.P.. Biorheology . 1990:27(34): 425-32. 3. Flow cytometric analysis of neutrophil subsets in thermally injured patients developing infection. Babcock, G.F., Alexander, J.W., Warden, G.d.. Clin. Immunol. Immunopathol. 1990 Ja;54(1):117-25. 4. Leukocyte Adhesion Deficiency: an inherited defect in Mac-1,LFA-1, and p150,95 glycoproteins. Anderson, D.C. and Springer, T.A.. Ann. Rev. Med. 1987;38:175. 5. Two pathways of CD11b/CD18-mediated neutrophil aggregation with different involvedment of protein kinase C-dependent phosphorylation. Merrill, J.T., Slade, S.G., Weissman, G., Winchester, R., Buyon, J.P.. J. Immunology. 1990 Oct.1;145(8):2608-15. 6. Tumor necrosis factor-induce degranulation in adherent human neutrophils is dependent on CD11b/CD18-integrin-triggered oscillations of cytosolic free Ca2+. Richter, J., Ng-Sikorski, J., Olsson, I., Andersson, T.. Proc. Natl. Acad. Sci. USA 1990 De;87(23):9472-6.

Protein Reference(s)

Database Name: UniProt

Accession Number: P11215

Safety Datasheet(s) for this product: