Catalogue

Background
The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subset of TGF beta -1 cells. This human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is expressed by 0.5 to 2 % of resting lymphocytes in Peripheral blood and lymphoid organs.

Synonyms: CD103

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
PBMC: Add 10 µl of MAB/106 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2° to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20° C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Cepek K., Wong D. Brenner M., and Springer T., Leukocyte Typing V val. G p 1666-67 (1995).

Protein Reference(s)

Database Name: UniProt

Accession Number: P38570

Safety Datasheet(s) for this product: