Catalogue
Mouse anti Human Calpain I, II (small subunit)
Catalog number: X1012$319.00
Add To CartClone | 28F3 |
Isotype | IgG1 |
Product Type |
Monoclonal Antibody |
Units | 100 µg |
Host | Mouse |
Species Reactivity |
Bovine Human |
Application |
Enzyme Immunoassay Immunoprecipitation Western Blotting |
Background
The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and µ-calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, µ-calpain is active at micromolar (µM) concentrations of Ca ++. In addition to the ubiquitously expressed m- and µcalpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
Synonyms: Mouse anti calpain I, II small subunit, p30
Source
Hybridoma produced by the fusion of splenocytes from mice immunized with human calpain with mouse myeloma.
Product
Provided as a concentration of 1 mg/ml in 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride, 50% glycerol, 3mM sodium azide.
Product Form: Unconjugated
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Applications
Use at 0.5-1 ug/ml for ELISA and western blot, 1-2ug/ml for immunoprecipitation.
Functional Analysis: Western Blotting
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Adamec, E., et al. 'Calpain I activation in rat hippocampal neurons in culture is NMDA receptor selective and not essential for excitotoxic cell death.' Brain Res. Mol. Brain Res. 1998, 54, 35-48
2. Guttmann, R.P. et al. 'Oxidation inhibits substrate proteolysis by calpain I but not autolysis.' J. Biol. Chem. 1997, 272, 2005-2012
Protein Reference(s)
Database Name: UniProt
Accession Number: P17655
Species Accession: Human
Safety Datasheet(s) for this product:
EA_Sodium Azide |
EA_Glycerol |