Catalogue

Background
Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighbouring cells. In addition, E-cadherin can interact heterophilically with integrin αEβ7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic Catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.

Source
MB2 is a Mouse monoclonal IgG2b antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with MCF-7/AZ cells expressing E-cadherin at their cell surface.

Product
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

Specificity
MB2 recognizes both the 120 kD E-cadherin and its 80 kD trypsin-resistant extracellular part. MB2 is a functional antibody in that it inhibits cell-cell adhesion.

Applications
MB2 is useful for flow cytometry, immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for flow cytometry and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent and 1:100 – 1:1000 for immunoblotting applications.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Bracke, M. E., Vyncke, B. M., Bruyneel, E. A., Vermeulen, S. J., De Bruyne, G. K., Van Larebeke, N. A., Vleminckx, K., Van Roy, F. M., and Mareel, M. M. (1993). Insulin-like growth factor I activates the invasion suppressor function of E-cadherin in MCF-7 Human mammary carcinoma cells in vitro, Br J Cancer 68, 282-9. 2. Steelant, W. F., Goeman, J. L., Philippe, J., Oomen, L. C., Hilkens, J., Krzewinski-Recchi, M. A., Huet, G., Van der Eycken, J., Delannoy, P., Bruyneel, E. A., and Mareel, M. M. (2001). Alkyl-lysophospholipid 1-O-octadecyl-2-O-methyl- glycerophosphocholine induces invasion through episialin-mediated neutralization of E- cadherin in Human mammary MCF-7 cells in vitro, Int J Cancer 92, 527-36. 3. Rong, H., Boterberg, T., Maubach, J., Stove, C., Depypere, H., Van Slambrouck, S., Serreyn, R., De Keukeleire, D., Mareel, M., and Bracke, M. (2001). 8-Prenylnaringenin, the phytoestrogen in hops and beer, upregulates the function of the E-cadherin/Catenin complex in Human mammary carcinoma cells, Eur J Cell Biol 80, 580-5.

Protein Reference(s)

Database Name: UniProt

Accession Number: P12830

Safety Datasheet(s) for this product: