Catalogue

Background
Desmin is a 53 kDa intermediate filament protein that exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

Source
D33 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified desmin from a leiomyosarcoma.

Product
Each vial contains 100ul 1mg/ml purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09% sodium azide

Formulation: Each vial contains 100ul 1mg/ml purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09% sodium azide

Specificity
The antibody D33 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma in human, hamster, rat and chicken. The antibody does not cross react with vimentin or any of the other intermediate filament proteins.

Applications
The D33 antibody is suitable for the detection of desmin by Western blotting and detects a band of 53kDa in muscle tissue extracts. The antibody is also suitable for the detection of desmin by immunocytochemistry on permeabilized cells, and Immunohistochemistry on frozen and formalin fixed, paraffin embedded tissues. The antibody works on paraffin-embedded material without pretreament with proteolytic enzymes, but the section should be pre-treated using heat mediated antigen retrieval with Tris-buffer containing 1 mM EDTA, pH 9,0 for 20 mins. Optimal antibody dilutions for the different applications should be determined by titration.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Van Muijen GN, Ruiter DJ, Warnaar SO. (1987) Coexpression of intermediate filament polypeptides in human fetal and adult tissues. Lab Invest. 57:359-69 2. 2. Pollock L, Rampling D, Greenwald SE, Malone M. (1995) Desmin expression in rhabdomyosarcoma: influence of the desmin clone and immunohistochemical method. J Clin Pathol. 48:535-8. 3. 3. Fetsch JF, Miettinen M, Laskin WB, Michal M, Enzinger FM. (2000) A clinicopathologic study of 45 pediatric soft tissue tumors with an admixture of adipose tissue and fibroblastic elements, and a proposal for classification as lipofibromatosis. Am J Surg Pathol. 24:1491-500. 4. 4. Chang TK, Li CY, Smithson WA. (1989) Immunocytochemical study of small round cell tumors in routinely processed specimens. Arch Pathol Lab Med.113:1343-8. 5. 5. Pohar-Marinsek Z, Srebotnik-Kirbis I. (2000) Desmin detection in FNAB samples of rhabdomyosarcoma: an immunocytochemical study. Cytopathology 11:171-8.

Protein Reference(s)

Database Name: UniProt

Accession Number: P17661

Safety Datasheet(s) for this product: