Catalogue

Background
This antibody is used for the detection of high risk Human Papilloma virus (HPV) infections in different tissues. It should always be used when the screening antibody has detected the presence of papillomavirus in suspected tissues. Human Papilloma virus type 16 and 18 are responsible for the development of cervical carcinoma, as well as carcinomas of the head and neck region and some other tumors.

Source
Mouse immunized with recombinant E6 protein fro HPV18

Immunogen: HPV type 18 E6 protein produced in bacteria

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 30 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002 if required.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 30 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002 if required.

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity
Human Papillomavirus Early Antigen E6 from HPV 16 and 18.

Applications
IHC(C, P)

Incubation Time: 60 min at RT

Working Concentration: (RTU) neat

Pre-Treatment: Improvement of staining with unmasking fluid G (Art. No. DE007) or unmasking fluid C (Art. No. DE000)

Positive Control: HPV-positive vulva carcinoma

Storage
2-8°C

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Cowsert et al. (1987) J. Natl. Cancer Inst. 79; 1053-1057. 2. Banks L.J. (1987) J. Gen. Virol. 68; 1351-1355. 3. Cason J., Patel D., Naylor J., et al. (1989) Identification of immunogenic regions of the major coat protein of human papillomavirus type 16 that contain type-restricted epitopes. J. General Virol. 70; 2973-2987. 4. Shepherd P.S., Lunny D., Brooks R., et al. (1992) The detection of human papilloma viruses in cervical biopsies by immunohistochemistry and in situ hybridization. Scand. J. Immunol. 36(11); 69-74.

Safety Datasheet(s) for this product: