Catalogue

Background
The reactivity of the antiserum is restricted to C1q. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C1q protein. Complexes containing C1q may also react with the antiserum. No reaction is obtained with C1r, C1s or any other plasma protein component or serum. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of C1q or C1q containing complexes in human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive non-precipitating antibody-binding techniques if proper controls are included.

Source
C1 component of human complement is a complex of three glycoproteins: C1q, C1r and C1s. C1q has a molecular weight of 492.000 and is present in plasma in an average concentration of 80 μg/ml. It consists of a complex of 18 polypeptide chains of three different types, A, B and C; 6 of each type. The subcomponent C1q is responsible for the binding of C1q to immuno-complexes an membranes via the Fc portion of the immunoglobulin. The subunit C1q is isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Product
Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.

Formulation: Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign

Specificity
Precipitating polyclonal Goat antiSerum to C1q fragment ofC1 component of Human complement

Species Reactivity: The antiSerum does not cross-react with any other component of Human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Applications
Precipitation assays.

Storage
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

Protein Reference(s)

Database Name: UniProt

Accession Number: P02746