Catalogue

Background
Protein serine/threonine phosphatase 4R1 is a protein which is associated with the catalytic subunit of protein serine/threonine phosphatase 4 (PP4C) and may possibly regulate the activity of the enzyme. PP4C is 65% identical to PP2AC at the amino acid level and has been placed in the type 2A family of phosphatases. PP4C has been highly conserved between species sharing 91% amino acid identity between human and Drosophila PP4C is predominantly localized in the nucleus in rat brain and liver but is most highly expressed in testis. Additionally, PP4C was demonstrated to be an essential enzyme in the development of Drosophila embryos. The expression of PP4C was reduced to 25% of the normal protein level in a mutant strain of Drosophila termed centrosomes minus microtubules (cmm). An interesting characteristic of the cmm phenotype is the presence of regions of cells that are unable to complete mitosis because no microtubules exist to connect chromatin and centrosomes. This phenotype implicates PP4C in the regulation of the nucleation and/or stabilization of microtubules. These data taken together indicate that PP4 has a crucial cellular function, although a physiological substrate for PP4 has not yet been identified.

Synonyms: Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 1; Protein Phosphatase X/A'2; PP4R1

Source

Immunogen: Antibody raised against synthetic peptide corresponding to amino acids 38 to 52 of the human protein phosphatase 4R1.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Detects protein phosphatase X/A (PP4 R1) protein by Western blot (5-10 µg/ml). Detects a 125 kDa band in bovine testes lysate. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Not available

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
Kloeker, S., et al. Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. J. Biol. Chem. 1999, 274, 5339-5347.

Protein Reference(s)

Database Name: UniProt

Accession Number: Q8TF05 (Human)Q8K2V1 (Mouse)Q8VI02 (Rat)

Species Accession: Human, Rat, Mouse

Safety Datasheet(s) for this product: