Accelerating the Pace of Discovery

Proteins and Peptides

Control antibodies are a vital part of assay design and ensure signal detected during antibody-based applications, can be attributed to specific antigen binding, rather than non-specific background staining.

Negative isotype controls function by determining the level of non-specific binding of the primary antibody to target. This is achieved by matching the control antibody host species, isotype, and if applicable fluorochrome, to that of the experimental specific antibody. A matched fluorochrome is required if the experimental primary antibody is directly conjugated. Negative control antibody is deliberately generated against a chemical or target not expressed by the cell or tissue of interest, so any binding will be non-specific in nature. This non-specific binding is most frequently caused by Fc receptors expressed on the surface of the target sample. These receptors bind the antibody’s Fc region independently of their Fab epitope binding domains.

Control antibodies are most commonly used in flow cytometry or immunohistochemistry to determine levels of background staining, but can also be utilized as blocking agents before addition of primary antibody.

Code Name Product Type Clone
P 106 Human IgG1 lamda G1m(f) Proteins & Peptides
P 182 Human IgG4 kappa Gm4a Proteins & Peptides
P 210 Human IgG1 lambda G1m (a) Proteins & Peptides
P 289 Human IgG3 lambda G3m (g) Proteins & Peptides
P 304 Human IgG4 kappa Gm4b Proteins & Peptides
P 315 Human IgG2 lambda G2m (n-) Proteins & Peptides
P 411 Human IgG3 kappa G3m (b) Proteins & Peptides
P 430 Human IgA1 kappa, polymer Proteins & Peptides
P 444 Human IgA lambda (dimer) Proteins & Peptides
P 532 Human IgG2 lambda G2m (n+) Proteins & Peptides
PO Horseradish peroxidase Proteins & Peptides
ShAlb Sheep Serum albumin Proteins & Peptides
X1632B Sphingosine Kinase 1 Blocking Peptide Blocking Peptide
X2050B Adiponectin Receptor 1 Blocking Peptide
X1098 Collagen Substrate, FITC Conjugate Proteins & Peptides N/A
X1211 Ral B Proteins & Peptides
X1213 Rap 2A Proteins & Peptides N/A
X1224B Sphingosine 1 Phosphate Receptor 1 (EDG-1) CT Blocking Peptide
X1227B Lysophosphatidic Acid Receptor 3 CT (EDG-7) Blocking Peptide
X1228B Sphingosine 1 Phosphate Receptor 5 CT (EDG-8) Blocking Peptide N/A
X1269B Lysophosphatidic Acid Receptor 1 CT (EDG-2) Blocking Peptide
X1312B Sphingosine 1 Phosphate 4 Receptor (CT) (EDG6) Blocking Peptide
X1573B Lysophosphatidic Acid Receptor 2 (EDG4) CT Blocking Peptide
X1642B Sphingosine Kinase 2 Blocking Peptide Blocking Peptide
X1644B Sphingosine 1-Phosphate Phosphatase 1 Blocking Peptide
X1645B Sphingosine 1-Phosphate Phosphatase 2 Blocking Peptide
X1646B GPR4 Blocking Peptide Blocking Peptide
X1852B FHL2 (SLIM3) Blocking Peptide
X1853B Sulf2 Blocking Peptide
X1854B SIAH1, E3 Ubiquitin Ligase Blocking Peptide
X1855B SPARC Blocking Peptide
X1868b Netrin 1 Blocking Peptide
X1869B Trag-3 Blocking Peptide
X1875B Sphingosine Kinase 1, Long Form, Rat Blocking Peptide
X1876B HDAC1, Human Blocking Peptide
X1877B Sphingosine Kinase 1 (Ser225), Mouse Blocking Peptide
X1878B Sphingosine Kinase 1, Mouse Blocking Peptide
X2294B Lass1 (Cross reacts with GDF1) Blocking Peptide
X2296B ACAD11 Blocking Peptide
X2299B Lass4 Blocking Peptide
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