Control antibodies are a vital part of assay design and ensure signal detected during antibody-based applications, can be attributed to specific antigen binding, rather than non-specific background staining.
Negative isotype controls function by determining the level of non-specific binding of the primary antibody to target. This is achieved by matching the control antibody host species, isotype, and if applicable fluorochrome, to that of the experimental specific antibody. A matched fluorochrome is required if the experimental primary antibody is directly conjugated. Negative control antibody is deliberately generated against a chemical or target not expressed by the cell or tissue of interest, so any binding will be non-specific in nature. This non-specific binding is most frequently caused by Fc receptors expressed on the surface of the target sample. These receptors bind the antibody’s Fc region independently of their Fab epitope binding domains.
Control antibodies are most commonly used in flow cytometry or immunohistochemistry to determine levels of background staining, but can also be utilized as blocking agents before addition of primary antibody.
Code | Name | Product Type | Clone |
---|---|---|---|
BAlb | Bovine Serum albumin | Proteins & Peptides | |
BGG | Bovine gamma globulin Fr.II | Proteins & Peptides | |
Bii-rGFP-100 | Purified recombinant Green Fluorescent Protein (wild type) | Proteins & Peptides | |
BSA | Bovine Serum albumin Fr.V | Proteins & Peptides | |
BSA/FITC | Bovine Serum albumin Fr.V, conjugated with FITC | ||
BSA/TRITC | Bovine Serum albumin Fr.V, conjugated with TRITC | ||
HSA | Human Serum albumin Fr.IV | Proteins & Peptides | |
HuAlb | Human Serum albumin | Proteins & Peptides | |
HuFab | Human Fab of normal IgG | Proteins & Peptides | |
P 016 | Human Bence Jones kappa | Proteins & Peptides | |
P 017 | Human Bence Jones lambda | Proteins & Peptides | |
P 020 | Human secretory IgA | Proteins & Peptides | |
MAlb | Mouse Serum albumin | Proteins & Peptides | |
MIgG | Mouse IgG | Proteins & Peptides | |
ShAlb | Sheep Serum albumin | Proteins & Peptides | |
X1632B | Sphingosine Kinase 1 Blocking Peptide | Blocking Peptide | |
X2050B | Adiponectin Receptor 1 | Blocking Peptide | |
X1098 | Collagen Substrate, FITC Conjugate | Proteins & Peptides | N/A |
X1211 | Ral B | Proteins & Peptides | |
X1213 | Rap 2A | Proteins & Peptides | N/A |
X1224B | Sphingosine 1 Phosphate Receptor 1 (EDG-1) CT | Blocking Peptide | |
X2051B | Adiponectin Receptor 2 | Blocking Peptide | |
X2067B | Serine Palmitoyltransferase 1 | Blocking Peptide | |
X2073B | Ceramide Glycosyltransferase | Blocking Peptide | |
X2287B | Sphingomyelin Phosphodiesterase 3 | Blocking Peptide | |
X2288B | LASS2 (Longevity Assurance Homolog 2) | Blocking Peptide | |
X2289B | SMase Activation Associated Factor | Blocking Peptide | |
X2291B | NUMB | Blocking Peptide | |
X2290B | Lass3 | Blocking Peptide | |
X2293B | APM2 (CT) | Blocking Peptide | |
X2292B | Lass1 | Blocking Peptide | |
X2295B | Monocarboxylate Transporter 3 (MCT3) | Blocking Peptide | |
X2294B | Lass1 (Cross reacts with GDF1) | Blocking Peptide | |
X2296B | ACAD11 | Blocking Peptide | |
X2299B | Lass4 | Blocking Peptide | |
X2302B | Lass6 | Blocking Peptide | |
X2312B | Sphingomyelin Synthase 1 (SMS1) | Blocking Peptide | |
X2335B | Roundabout homolog 1, ROBO1 | Blocking Peptide | |
X2337B | FBXW7 | Blocking Peptide | |
X2339B | Sodium/hydrogen exchanger 1 | Blocking Peptide |