Accelerating the Pace of Discovery

Secondary Antibodies

Secondary antibodies are valuable biological tools that are employed extensively in research applications and diagnostic assays. Secondary antibodies are conjugated to reporter molecules, such as enzymes or fluorochromes, which provide a signal that can be detected and measured. They are commonly used in antibody based applications to detect an antigen specific primary antibody that is not already conjugated to a reporter. Applications that may require a secondary antibody include immunohistology, immunofluorescence, western blotting, enzyme linked immunosorbent assays (ELISA), flow cytometry and lateral flow assays. Careful selection of suitable secondary antibodies also allows researchers to perform simultaneous labelling of multiple targets in a single sample.

A diverse range of monoclonal (Mab), polyclonal (PAb) secondary antibodies are available that vary according to the host, in which the antibody was raised. They are also grouped according to characteristics of the primary antibody recognised with respect to animal species, antibody class and region of the antibody. Secondary antibodies may also be supplied as whole antibodies or as antibody fragments, which can provide technical advantages in some applications.

In addition, antibodies can be conjugated to numerous different reporter molecules thus providing researchers and assay developers with enormous application and assay design flexibility. The correct selection of an appropriate secondary antibody is vital to the success of any immunostaining technique.

Exalpha manufactures and supplies polyclonal secondary antibodies that have been raised in goats and are specific for the Fc portion of mouse IgG. The secondary antibodies recognise all subclasses of mouse IgG and are available conjugated to fluorescein isothiocyanate (FITC) or tetramethylrhodamine isothiocyanate (TRITC). The antibodies are suitable for use in immunocytochemistry, immunofluorescence, immunohistochemistry and ELISA applications.

In addition, Exalpha offers an extensive range of high quality polyclonal secondary antibodies that are suitable for the detection of mouse and rat immunoglobulin classes and subclasses. The range includes secondary antibodies conjugated to horseradish peroxidase, biotin, FITC or TRITC. The antibodies have been tested for Ig cross reactivity and are designed for use in a wide range of research applications.

Code Name Product Type Clone
R/PAP Rabbit peroxidase-anti-peroxidase Polyclonal
R7AG/IgG(H+L)/Bio Rabbit anti Goat IgG (heavy and light chains) (non Human and Mouse), conjugated with Biotin Polyclonal
R7AG/IgG(H+L)/FITC Rabbit anti Goat IgG (heavy and light chains) (non Human and Mouse), conjugated with FITC Polyclonal
R7AG/IgG(H+L)/PO Rabbit anti Goat IgG (heavy and light chains) (non Human and Mouse), conjugated with Horseradish peroxidase Polyclonal
Ra/PAP Rat peroxidase-anti-peroxidase Polyclonal
RAB/IgG(H+L) Rabbit anti Bovine IgG (heavy and light chains) Polyclonal
RAB/IgG(H+L)/7S Rabbit anti Bovine IgG (heavy and light chains) Polyclonal
RAB/IgG(H+L)/Bio Rabbit anti Bovine IgG (heavy and light chains), conjugated with Biotin Polyclonal
RAB/IgG(H+L)/FITC Rabbit anti Bovine IgG (heavy and light chains), conjugated with FITC Polyclonal
RAB/IgG(H+L)/PO Rabbit anti Bovine IgG (heavy and light chains), conjugated with Horseradish peroxidase Polyclonal
RAB/IgG(H+L)/TRITC Rabbit anti Bovine IgG (heavy and light chains), conjugated with TRITC Polyclonal
RAB/IgG1 Rabbit anti Bovine IgG1 (subclass specific) Polyclonal
RAB/IgG2 Rabbit anti Bovine IgG2 (subclass specific) Polyclonal
RAB/IgM(Fc) Rabbit anti Bovine IgM (Fc specific) Polyclonal
RAB/IgM(Fc)/7S Rabbit anti Bovine IgM (Fc specific) Polyclonal
RAB/IgM(Fc)/Bio Rabbit anti Bovine IgM (Fc specific), conjugated with Biotin Polyclonal
RAB/IgM(Fc)/FITC Rabbit anti Bovine IgM (Fc specific), conjugated with FITC Polyclonal
RAB/IgM(Fc)/PO Rabbit anti Bovine IgM (Fc specific), conjugated with Horseradish peroxidase Polyclonal
RAB/sIgA Rabbit anti Bovine secretory IgA Polyclonal
RACa/IgA(Fc) Rabbit anti Cat IgA (Fc specific) Polyclonal Antibody Polyclonal
RACa/IgG(Fc) Rabbit anti Cat IgG (Fc specific) Polyclonal Antibody Polyclonal
RACa/IgG(H+L) Rabbit anti Cat IgG (heavy and light chains) Polyclonal
RACa/IgG(H+L)/Bio Rabbit anti Cat IgG (heavy and light chains), conjugated with Biotin Polyclonal
RACa/IgG(H+L)/FITC Rabbit anti Cat IgG (heavy and light chains), conjugated with FITC Polyclonal
RACa/IgG(H+L)/PO Rabbit anti Cat IgG (heavy and light chains), conjugated with Horseradish peroxidase Polyclonal
RACh/Ig Rabbit anti Chicken IgG IgA IgM (heavy and light chains) Polyclonal
RACh/IgG(H+L) Rabbit anti Chicken IgG (heavy and light chains) Polyclonal
RACh/IgG(H+L)/7S Rabbit anti Chicken IgG (heavy and light chains) Polyclonal
RACh/IgG(H+L)/Bio Rabbit anti Chicken IgG (heavy and light chains), conjugated with Biotin Polyclonal
RACh/IgG(H+L)/FITC Rabbit anti Chicken IgG (heavy and light chains), conjugated with FITC Polyclonal
RACh/IgG(H+L)/PO Rabbit anti Chicken IgG (heavy and light chains), conjugated with Horseradish peroxidase Polyclonal
RAD/IgA(Fc) Rabbit anti Dog IgA (Fc specific) Polyclonal
RAD/IgG(H+L) Rabbit anti Dog IgG (heavy and light chains) Polyclonal Antibody Polyclonal
RAD/IgG(H+L)/7S Rabbit anti Dog IgG (heavy and light chains) Polyclonal Antibody Polyclonal
RAD/IgG(H+L)/Bio Rabbit anti Dog IgG (heavy and light chains), conjugated with Biotin Polyclonal
RAD/IgG(H+L)/FITC Rabbit anti Dog IgG (heavy and light chains), conjugated with FITC Polyclonal
RAD/IgG(H+L)/TRITC Rabbit anti Dog IgG (heavy and light chains), conjugated with TRITC Polyclonal
RAD/IgG(H+L)/PO Rabbit anti Dog IgG (heavy and light chains), conjugated with Horseradish peroxidase Polyclonal
RADu/Ig Rabbit anti Duck IgG IgA IgM (heavy and light chains) Secondary Antibodies Polyclonal
RADu/IgG(H+L) Rabbit anti Duck IgG (heavy and light chains) Polyclonal Antibody Polyclonal
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