Catalogue

Background
c-erbB-2 is a receptor tyrosine kinase. It exhibits extracellular domains with two cysteine-rich sequences, and a cytoplasmic tyrosine kinase domain flanked by large hydrophilic tails that carry several tyrosine autophosphorylation sites. Approximately 25% of primary breast and ovarian tumors were found to overexpress the protein.

Synonyms: p185erbB2, MLN 19, CD340, c-ErbB-2

Source

Immunogen: Full-length recombinant c-erbB-2/HER2/neu protein.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Breast carcinomas

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Stancovski, I et. al. 'Mechanistic aspects of the opposing effects of monoclonal antibodies to the ERBB2 receptor on tumor growth.' Proc. Natl. Acad. Sci USA, 1991, 88(19):8691-5. 2. Hurwitz E et. al. 'Suppression and promotion of tumor growth by monoclonal antibodies to ErbB-2 differentially correlate with cellular uptake.' Proc. Natl. Acad. Sci USA, 1995, 92(8):3353-7.

Protein Reference(s)

Database Name: UniProt

Accession Number: P04626

Safety Datasheet(s) for this product: