Catalogue

Background
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.

Synonyms: sheep anti cytochrome c, cyto c, cytochrome-c

Source

Immunogen: Full length recombinant Cytochrome C

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Applications: Western Blot 1:5,000, shows a MW of 14.4kDa. May be used in immunofluorescence applications with human and rat cells; Immunoprecipitation - Empirical.

Functional Analysis: Western Blotting

Positive Control: Rat1 cell line; Mitochondrion matrix

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Morison, I.M., et al. 'A mutation of human cytochrome c enhances the intrinsic apoptotic pathway but causes only thrombocytopenia.' Nat. Genet. 2008, 40, 387-389 2. Skulachev, V.P. 'Cytochrome c is the apoptotic and antioxidant cascades.’ FEBS Lett 1998, 423, 275-280

Protein Reference(s)

Database Name: UniProt

Accession Number: P99999 (Human)P62898 (Rat)P00008 (Rabbit)P00011

Species Accession: Human, Rat

Safety Datasheet(s) for this product: