Catalogue

Background
Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to Icurrent in heart and current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits In response to membrane depolarization, Kv4.2 channels accumulate in the closed-inactivated state(s), from which they directly recover, bypassing the open state. Kv4.2 is a substrate for ERK in vitro and in vivo, and suggest that ERK may regulate potassium-channel function by direct phosphorylation of the pore-forming alpha subunit.

Synonyms: RK5, Shal1, Kcnd2, Potassium voltage-gated channel subfamily D member 2

Source
Rabbits were immunized with a peptide representing amino acids 453-470 of rat Kv4.2 conjugated to KLH.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Optimal antibody concentration should be evaluated by serial dilutions. Suggested Western Blot dilution 1:1000. Other applications: Immunoprecipitation, immunofluorescence.

Functional Analysis: Western Blotting

Positive Control: Highly expressed in heart and throughout the brain, with similar levels in cortex and hypothalamus, and much higher levels in hippocampus, dentate gyrus and the habenular nucleus of the thalamus. Detected at similar levels in heart atrium and ventricle. Detected in aorta, cardiac and smooth muscle.

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Roberds,S.L. et.al, Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart. Proc. Natl. Acad. Sci. U.S.A. 88 (5), 1798-1802 (1991) 2. Bahring, K.et.al. Kinetic analysis of open- and closed-state inactivation transitions in human Kv4.2 A-type potassium channels. J Physiol ;535(Pt 1):65-81 (2001) 3. Adams, T. et.al,The A-type potassium channel Kv4.2 is a substrate for the mitogen-activated protein kinase ERK. J Neurochem 5(6):2277-87 (2000)

Protein Reference(s)

Database Name: UniProt

Accession Number: Q63881

Species Accession: Rat

Safety Datasheet(s) for this product: