Catalogue

Background
Zinc Finger Protein GLI1 is a member of the Kruppel family of zinc finger proteins. It is thought that it may regulate the transcription of specific genes during normal development and may play a role in craniofacial development and digital development. It also may be involved in the development of the central nervous system and gastrointestinal tract. This protein mediates SHH signaling and thus cell proliferation and differentiation. It has been localized to the cytoplasm and nucleus. It is tethered in the cytoplasm by binding to SUFU and activation and translocalization to the nucleus is promoted by interation with STK36. Protein expression is amplified in glioblastoma cells. Defects in the GLI1 protein may be a cause of breast cancer.

Synonyms: Glioma-associated oncogene; Oncogene GLI

Source

Immunogen: Synthetic peptide derived from human zinc finger protein GLI1.

Product

Product Form: Affinity Purified

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: Lot specific, see vial

Applications
Antibody can be used for Western blotting (1:400 starting dilution). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Protein found in testis, myometrium and fallopian tube.

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Kinzler, K.W., et al. 'The GLI gene is a member of the Kruppel family of zinc finger proteins.; Nature 332:371-374(1988). 2. Wang, Z.Q., et al. 'Identification of polymorphic variants of the GLI1 oncogene.; J. Invest. Dermatol. 115:328-329(2000).

Protein Reference(s)

Database Name: UniProt

Accession Number: P08151 (Human)

Species Accession: Human

Safety Datasheet(s) for this product: